RASSF1-AS1, which was transcribed from the opposite strand on the RASSF1 gene locus in 3p21.3 of 790bp, recruits PRC2 to the RASSF1A promoter, reducing the expression of RASSF1A and increasing cell proliferation.
RASSF1-AS1:RASSF1 antisense RNA 1(HGNC nomenclature)
ANRASSF1, an endogenous unspliced long noncoding RNA (lncRNA) that is transcribed from the opposite strand on the RASSF1 gene locus through RNA polymerase II ,is 5′-capped and polyadenylated and exhibits nuclear localization and has a shorter half-life compared with other lncRNAs that bind PRC2.
It belongs to the category of "Antisense" in lncRNA classification.
It exhibits nuclear localization.
ANRASSF1 formed an lncRNA/DNA hybrid, which mediated the recruitment of SUZ12, a member of PRC2, to the RASSF1A promoter.The recruitment of SUZ12 resulted in a marked increase in the H3K27me3 levels only at the RASSF1A promoter region, without accumulation of the repressive mark either at the RASSF1C promoter or the four neighboring loci. ANRASSF1-mediated gene repression occurred in a highly location-specific manner, as only the RASSF1A isoform, which overlaps the antisense transcript, was affected. .
ANRASSF1 expression is significantly higher in triple negative sample, which suggests a putative role in breast cancer and implies that it can be used as a potential cancer biomarker.
ANRASSF1 endogenous expression is higher in breast and prostate tumor cell lines compared with non-tumor, and an opposite pattern is observed for RASSF1A.
ANRASSF1 overexpression causes a marked increase in both PRC2 occupancy and histone H3K27me3 repressive marks, specifically at the RASSF1A promoter region.
|Assay||Primer||Forward primer||Reverse primer|
|Strand specific RT||ANRASSF1||5'-CCGCGCAGAATTAGCCTCT-3'||5'-CCAATGAGGAAAGGGGAAGT-3'|
|Cloning (overexpression and promoter assay)||Antisense-ANRASSF1||TGGGTACCGCAGCGGGTGGAGTACTTG||5'-TGAAGCTTCCAATGAGGAAAGGGGAAGT-3'|
- Breast cancer.
>gi|565671811|ref|NR_109831.1| Homo sapiens RASSF1 antisense RNA 1 (RASSF1-AS1), long non-coding RNA
Labs working on this lncRNA
- Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brasil.
- Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
- Beckedorff FC, Ayupe AC, Crocci-Souza R, Amaral MS, Nakaya HI, Soltys DT, et al. The intronic long noncoding RNA ANRASSF1 recruits PRC2 to the RASSF1A promoter, reducing the expression of RASSF1A and increasing cell proliferation[J]. PLoS genetics. 2013,9(8):e1003705.
- Iranpour M, Soudyab M, Geranpayeh L, Mirfakhraie R, Azargashb E, Movafagh A, et al. Expression analysis of four long noncoding RNAs in breast cancer[J]. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2016,37(3):2933-40.