GHET1, which is located in an intergenic region on chromatin 7, acting as a prognostic factor for gastric carcinoma that promotes c-Myc mRNA stability and expression and the growth of gastric carcinoma functions as an oncogene in gastric carcinoma.
GHET1: gastric carcinoma proliferation enhancing transcript 1 (HGNC nomenclature)
"gastric carcinoma high expressed transcript 1", lncRNA-GHET1 
GHET1, gastric carcinoma proliferation enhancing transcript 1, located in an intergenic region on chromatin 7.
GHET1 is up-regulated in gastric carcinoma tissues in comparison with adjacent normal gastric tissues, and GHET1 up-regulation is correlated with tumor size and invasion. High GHET1 expression in gastric carcinoma tissues indicates poor overall survival of gastric carcinoma patients. Enhanced GHET1 expression promotes proliferation of gastric carcinoma cells in vitro and in vivo, while inhibition of GHET1 expression inhibits gastric carcinoma cell proliferation , which indicates that GHET1 functions as an oncogene in gastric carcinoma.
GHET1 associates with IGF2BP1 and enhances the physical interaction between c-Myc mRNA and IGF2BP1 protein.
- gastric carcinoma
GHET1 expression levels were significantly increased in gastric carcinoma tissues compared with adjacent normal gastric tissues.
siRNAs specifically targeting GHET1 were synthesized by Invitrogen, siRNAs specifically targeting c-Myc were synthesized by GenePharma (Shanghai, China), and siRNAs specifically targeting IGF2B1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
For each sample, gene expression was normalized to 18S rRNA. The primer sequences used were as follows:
|Primer||Forward primer||Reverse primer|
>gi|732170727|ref|NR_130107.1| Homo sapiens gastric carcinoma proliferation enhancing transcript 1 (GHET1), long non-coding RNA
Labs working on this lncRNA
- Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China .
- Yang F, Xue X, Zheng L, Bi J, Zhou Y, Zhi K, et al. Long non-coding RNA GHET1 promotes gastric carcinoma cell proliferation by increasing c-Myc mRNA stability[J]. The FEBS journal. 2014,281(3):802-13.