CAHM(Colorectal Adenocarcinoma HyperMethylated), which is located about 730bp upstream of the adjacent gene, QKI, shows specially hypermethylated in colorectal neoplasia.
CAHM: colon adenocarcinoma hypermethylated(non-protein coding)(HGNC nomenclature)
LINC00468, "long intergenic non-protein coding RNA 468"
CAHM is located adjacent to the gene QKI.
CAHM shows specially hypermethylated in colorectal neoplasia compared with lung, prostate and breast cancer.
The levels of hypermethylated CAHM of CRC patients are increased in blood than normals. Moreover, the increaseing levels of mythylated CAHM is correlated with advancing disease stage. According to these results, CAHM may act as a potential marker for CRC substypes.
It has been found that CAHM and its neighboring gene QKI are both downregulated in colorectal neoplasia because of hypermethylation, which suggests their high positive correlation.
|Input Material||Gene targets||Oligonucleotides|
|BisDNA||CAHM|| Region A
A1:5’-ATt TGT AAA AAT GTT GAt TTt TGt TTT TtA Gat-3’ A2:5’-TCT TaT TaC ACC TTC CCR TTA TTC Ta-3’
|CAHM|| Region B
B1:5’-GtY GTG tTG tTT Ttt AGt tTt TtA GtA AAT t-3' B2:5’-CAC RaT aCR aAa aaC Taa Taa aCT TTC CTT a-3’
|Bisulphite conversion dependent quantitative PCR|
|BisDNA||CAHM|| (within Region A)
1:5’-GAA GGA AGt AAT TCG AGt ACG AtT GAC-3’ 2:5’-CCC gaA CGC AaC GaC TTA a-3’
|cDNA||CAHM||2.1:5’-AGG GGA GCG TCA GTC GTG CT-3 ’
2.2:5’-TGC GGC TTC ATT CCC TCA CGG-3’
- colorectal adenocarcinoma
Labs working on this lncRNA
- Clinical Genomics Pty Ltd; North Ryde, NSW Australia..
- Pedersen SK, Mitchell SM, Graham LD, McEvoy A, Thomas ML, Baker RT, et al. CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia[J]. Epigenetics : official journal of the DNA Methylation Society. 2014,9(8):1071-82.