BDNF-AS which is approximately 200 kb downstream from the BDNF promoter and is located on the positive strand of chromosome-11,represses BDNF sense RNA transcription.
BDNF-AS:BDNF antisense RNA 1 (HGNC nomenclature)
BDNF-AS1, BT2A, BT2B, BT2C, BT2D, NCRNA00049, "non-protein coding RNA 49" 
LncBook ID: HSALNT0288893.
BDNF-AS is approximately 200 kb downstream from the BDNF promoter and it is located on the positive strand of chromosome-11. Transcription from this site gives rise to 16–25 splice variant long ncRNAs with 6–8 exons8. Exon-5 of BDNF-AS, which contain 225-nucleotides of full complementarity to BDNF mRNA (overlapping) and exon-4 (non-overlapping) are common between all these variants . 
It belongs to the category of "Antisense" in lncRNA classification.
BDNF-AS tonically represses BDNF sense RNA transcription by altering chromatin structure at the BDNF locus, which in turn reduces endogenous BDNF protein and function.
BDNF-AS represses chromatin by recruiting Polycomb Repressive Complex 2 (PRC2) to the BDNF promoter region.
BDNF-AS siRNA induces activation of the BDNF–TrkB–PI3K/Akt pathway following hypoxia/reoxygenation(H/R)-induced neurotoxicity.
BDNF-AS mRNA levels are generally 10–100 fold higher than BDNF-AS transcript, except in testis, kidney, and heart, which contain equal or higher levels of BDNF-AS. Both transcripts are expressed in brain, muscle and embryonic tissues. BDNF mRNA levels were relatively low in all post-natal tissues examined except in brain, bladder, heart, and skeletal muscle. In rhesus monkey and mouse tissues, both transcripts are co-expressed in many tissues, which suggest BDNF-AS potential for regulation of BDNF mRNA.
BDNF expression was significantly downregulated in patients with cerebral infarction, whereas the expression of BDNF-AS was significantly upregulated. In both human cortical neurons (HCN2) and human astrocytes, H/R significantly induced the expression of BDNF-AS, but significantly decreased BDNF expression.
|Human BDNF-AS siRNA-1||GGCTCACCAGTTGTTTGTT||siRNA|
|Scramble siRNA-1||AGCTCGCCAGTCGTTTATT||Scrambled control|
|Human BDNF-AS siRNA-2||GCAATGTATCTTAGGCTCA||siRNA|
|Human BDNF-AS siRNA-3||GCTAATCTTACAACAGCAC||siRNA|
|Scramble siRNA-3||ACTAAGCTTACAGCAGCGC||Scrambled control|
|Human BDNF-AS siRNA-4||TCCCTACAAACATGTCAT||siRNA|
|Scramble siRNA-4||GCCCGACAAACAAGTCAAT||Scrambled control|
|Control siRNA||CCUCUCCACGCGCAGUACATT||Non-targeting control|
|Human BDNF-AS primer-F||AGTGGCTAATATTACAACAGCACAA||Real time PCR|
|Human BDNF-AS primer-R||CTCAGTAGTCAAGTGCCTTTGGA||Real time PCR|
|Human BDNF-AS probe||CCTCCTCTTCTCTTTCTGGTTAG||Real time PCR|
Labs working on this lncRNA
- Department of Psychiatry and Behavioral Sciences and Center for Therapeutic Innovation .
- Department of Neurology, Guangzhou Zengcheng People’s Hospital and Boji Hospital of Sun Yat-Sen University, Guangzhou, People’s Republic of China.
- Modarresi F, Faghihi MA, Lopez-Toledano MA, Fatemi RP, Magistri M, Brothers SP, et al. Inhibition of natural antisense transcripts in vivo results in gene-specific transcriptional upregulation[J]. Nature biotechnology. 2012,30(5):453-9
- Zhong J B, Li X, Zhong S M, et al. Knockdown of long noncoding antisense RNA brain-derived neurotrophic factor attenuates hypoxia/reoxygenation-induced nerve cell apoptosis through the BDNF–TrkB–PI3K/Akt signaling pathway[J]. Neuroreport, 2017, 28(14): 910-916.