NONHSAT129019
PRNCR1(PCNCR1), is pervasively transcribed from the 8q24 “gene desert” region in strong association with susceptibility of prostate cancer.
Contents
Annotated Information
Name
PRNCR1: prostate cancer associated non-coding RNA 1(HGNC nomenclature)
pubmedID
20874843 23945587 24330491
Disease
Prostate Cancer
Characteristics
PRNCR1(prostate cancer non-coding RNA 1) , ∼13 kb intron-less long non-coding RNA (ncRNA) ,was transcribed in centromeric region (so-called Region 2: Chr8: 128.14-128.28 Mb) on 8q24 hat ,a large “gene-desert” ,whose region is susceptibility to prostate cancer[1].
PRNCR1 (also known as PCAT8) and PCGEM1, bind successively to the androgen receptor and strongly enhance both ligand-dependent and ligand-independent androgen-receptor-mediated gene activation programs and proliferation in prostate cancer cells[2].
Function
PRNCR1 overexpression can potentially serve as a required component of castration-resistance in prostatic tumors[2].
the polymorphisms in the lncRNA PRNCR1 may be related to the development of CRC, offering a novel and potential strategy for functional analysis of susceptibility loci to human diseases[3].
Diseases
Expression
PRNCR1 expression was upregulated in some of the PC cells as well as precursor lesion prostatic intraepithelial neoplasia[1].
The primers for real-time quantitative PCR were as follows,
| Primer | Forward | Reverse |
|---|---|---|
| PRNCR1 | 5'-CCAGGG-GGAAACACACAG-3' | 5'-AAATGGCAGTTTCCTTCAATG-3'[1] |
| β-actin (ACTB) | 5'-TTGGCTTGACTCAGGATTTA-3' | 5'-ATGCTATC-ACCTCCCCTGTG-3'[1] |
siRNA target sequences of were as follows,:
| Primer | Sequences |
|---|---|
| si1 | 5'-GCUCUUAAGGAAAUAACUU-3'[1] |
| si2 | 5'-GACGGUUCUGUUUAAGUAA-3'[1] |
| siEGFP(as a negative control) | 5'-GAAGCAGCACGACUUCUUC-3'[1] |
The knockdown effect was measured by RT-PCR using the primer set: 5′-ATTGTTCCCAGTTCTCTGGAC-3′ and 5′-GGAGGAAGTGGAGTAGGTATAAGAG-3′.
Allelic Information and Variation
The transcript is an approximately 13-kb intron-lessnon-coding RNA spanning a region between rs1016342 andrs7463708, and named this novel ncRNA as PRNCR1(prostate cancer non-coding RNA 1, GenBank Accession no.AB458446).[1]
Labs working on this lncRNA
- Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan[1].
- Howard Hughes Medical Institute, Department of Medicine, University of California San Diego, La Jolla, California 92093, USA[2].
- Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children's Health; Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P,R, China[3].
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Chung S, Nakagawa H, Uemura M, Piao L, Ashikawa K, Hosono N, et al. Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility[J]. Cancer science. 2011,102(1):245-52.
- ↑ 2.0 2.1 2.2 2.3 Yang L, Lin C, Jin C, Yang JC, Tanasa B, Li W, et al. lncRNA-dependent mechanisms of androgen-receptor-regulated gene activation programs[J]. Nature. 2013,500(7464):598-602.
- ↑ 3.0 3.1 3.2 Li L, Sun R, Liang Y, Pan X, Li Z, Bai P, et al. Association between polymorphisms in long non-coding RNA PRNCR1 in 8q24 and risk of colorectal cancer[J]. Journal of experimental & clinical cancer research : CR. 2013,32:104.
Basic Information
| Transcript ID |
NONHSAT129019 |
| Source |
NONCODE4.0 |
| Same with |
PRNCR1, |
| Classification |
intergenic |
| Length |
1248 nt |
| Genomic location |
chr8-:128098508..128099755 |
| Exon number |
1 |
| Exons |
128098508..128099755 |
| Genome context |
|
| Sequence |
000001 CAGCAGAGAC GCCAATACAG ACGACAAAAC CAGCAGTCTT CATCTGACTC TGGTTCCTCC TTCTCCTCAG AAGATGAACG 000080
000081 ACCCAAGAGA TCCCATGTGA AGAATGGTGA GGTTGGCAAG CGGCGGAGAC ATTCCCCTTC CCGGAGTGCT TCTCCATCAC 000160 000161 CACGAAAGCA CCAAAAAGAG ACTTCCCCTC ATGGTAGACG GAGGAGAAGT CCATCCCCAC CACCCACCAG AAGGCGACGG 000240 000241 TCTCCTTCTC CCGCCCCTCA TCCTCGACGG CTCAGGACTC CCACACCACC ACCACCACGA CGAAGGACTC CTTCTCCTCC 000320 000321 CCTACGTCGG CGCTCACCTT CTCCTAGAAG ATACTCTCCT CCAATACAGA GGAGATACTC TCCTTCTCCA CCTCCAAAGA 000400 000401 GAAGAACGGC TTCACCTCCT CCCCCTCCTA AACGAAGAGC ATCACCATTT CCACCACCAA AGCGGCGGGT CTCCCATTCT 000480 000481 CCACCTTCCA AAGAAGCTCC CCAGTCACCA AGAGACGTTC ACCTTCACTA TCATCCAAGC ATAGGAAAGG GTCTTCCCCA 000560 000561 AGCCGCTCTA CCCGGGAGGC CCGATCACCG CATCCAAACA AATGGCATTC GCCCTCATCA CGGCCTCGAG CTCCTCAGAC 000640 000641 CTCAAGTCCT CCACCCGTTC GAAGAGGAGC GTCGTCATCA CCCCAAAGAA GGCAGTCCCC GTCTCCAAGT ACTAGGCCCA 000720 000721 TTAGGAGAGT CTCCAGGACT CCGGAACCTA AAAAGATAAA AAAAGGCTGC TTCCCCAAGC CCACAGTCTG TAAGGAGGGT 000800 000801 CTCATCCTCC CGATCTGTCT CTGGGTCTCC TGAGCCAGCA GCTAAAAAGC CCCCAGCATC TCCATCCCCC ATCCAGTCTC 000880 000881 AGTCACCGTC TACAAACTGG TCACCAGCTG TACCGGTCAA AAAGGCCAAA AGCCCAACAC CGAGCCCATC ACTGCCAAGA 000960 000961 AATTCAGATC aagaaggaga tggaaagaaa aagaagaaaa agaaggacag gaaacacaaa aaggataaga agcacaagaa 001040 001041 gcacaaaaaa cacaagaagg aaaaggctgt ggctgcagct gctgcagctg ctgtgacccc tgcagccatt gcagctgcCA 001120 001121 CAACCACATT AGCACAGGAA GAGCCAGTGG CAGCGCCAGA GCCGAAGAAG GAGACTGAAA GTGAAGCTGA AGATAACCTT 001200 001201 GATGATTTAG AAAAGCACCT GCGTGAAACG GCCCCGAGAT CAATGAGG |
