Difference between revisions of "NONHSAT091315"

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Please input one-sentence summary here.
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''TUSC7'' is a p53-regulated tumor suppressor long noncoding gene, which acts in part through repression of miR-211
 
 
 
==Annotated Information==
 
==Annotated Information==
 
===Name===
 
===Name===
''TUSC7'': Tumor suppressor candidate 7 (HGNC nomenclature), LOC285194 <ref name="ref1" />.
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''TUSC7'': Tumor suppressor candidate 7 (HGNC nomenclature), also known as LOC285194, LSAMP antisense RNA 3 <ref name="ref1" /><ref name="ref4" />.
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Approved symbol: RN7SK
  
===Characteristics===
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Approved name: TUSC7
Together with the noncoding RNA BC040587 and the tumor suppressor gene LSAMP (limbic system-associated membrane protein), LOC285194 is present in the focal region of chr3q13.31 (osteo3q13.31), which is the most altered region in osteosarcomas. These genes are proposed to be cooperatively acting tumor suppressors <ref name="ref1" />.
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HGNC ID: HGNC:27701
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Previous names: non-protein coding RNA 295; LSAMP antisense RNA 3; tumor suppressor candidate 7 (non-protein coding)
  
===Features===
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Previous symbols: NCRNA00295; LSAMP-AS3
  
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Alias symbols: LINC00902; LOC285194
  
Recurrent focal copy-number changes and loss of heterozygosity (LOH), which usually involves loss of LOC285194 and BC040587 expression, implicate these ncRNAs in ostecosarcoma. Focal osteo3q13.31 CNAs and LOH are also common in cell lines from several other cancers (such as lung, autonomic and central nervous system, blood, endometrium, soft tissue, skin, gastrointestinal tract, urinary tract, and breast).
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RefSeq ID: NR_015391
  
Depletion of LOC285194 by siRNA promoted proliferation of normal osteoblasts (with a mild increase in the G1 population) by cell-cycle transcripts (Cyclin D1, VEGF, and VEGFR1 up-regulation, and cyclin A2 and cyclin B1 suppression) as well as regulation of apoptotic genes (such as suppression of BCL2 and BimEL pro-apoptotic genes).
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LncBook ID: [https://bigd.big.ac.cn/lncbook/transcript?transid=HSALNT0059175 HSALNT0059175]
  
Genetic deletions of LOC285194 or BC040587 in tumor DNA were also associated with dramatic decrease in survival of osteosarcoma patients.
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===Characteristics===
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''TUSC7''  is four exon transcript of 2105 bp long noncoding RNA, located on the focal region of human chromosome chr3q13.31 (osteo3q13.31) <ref name="ref1" /><ref name="ref4" />. Exon 4 harbors two miR-211 binding sites <ref name="ref4" />.  
  
 
===Function===
 
===Function===
Please input function information here.
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Together with the other noncoding RNA ''BC040587'' and the tumor suppressor gene ''LSAMP'' (limbic system-associated membrane protein) present in focal region (osteo3q13.31), ''TUSC7'' cooperatively acting as tumor suppressor genes <ref name="ref1" />.
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Depletion of ''TUSC7'' by siRNA promoted proliferation of normal osteoblasts (with a mild increase in the G1 population) by cell-cycle transcripts (Cyclin D1, VEGF, and VEGFR1 up-regulation, and cyclin A2 and cyclin B1 suppression) as well as regulation of apoptotic genes (such as suppression of BCL2 and BimEL pro-apoptotic genes) <ref name="ref1" />.
  
 
===Expression===
 
===Expression===
Please input expression information here.
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''TUSC7'' is significantly decreased in Colorectal cancer tissues <ref name="ref2" /><ref name="ref3" />.
 
 
===Conservation===
 
Please input conservation information here.
 
  
===Misc===
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''TUSC7'' is downregulated in osteosarcoma  <ref name="ref1" />.
Please input misc information here.
 
  
===Transcriptomic Nomeclature===
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{| class='wikitable' style="text-align:center"
Please input transcriptomic nomeclature information here.
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|-
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! | Experiment
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! | Forward primer
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! | Reverse primer
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|-
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| rowspan="1"|qRT-PCR
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| | 5’-GGAAACAGAAGGCACCTCA-3’
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| |5’-TCTCAGAGGTCAAACAGGCA-3’<ref name="ref3" />
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|}
  
 
===Regulation===
 
===Regulation===
Please input regulation information here.
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''p53'' transcriptionally regulates the ''TUSC7'' by direct binding to its promoter region <ref name="ref4" />.
 
 
===Allelic Information and Variation===
 
Please input allelic information and variation information here.
 
  
 
===Disease===
 
===Disease===
* Colorectal cancer <ref name="ref2" />
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* Colorectal cancer <ref name="ref2" /><ref name="ref3" />
* Osteosarcoma <ref name="ref" />
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* Osteosarcoma <ref name="ref1" />
  
 
==Labs working on this lncRNA==
 
==Labs working on this lncRNA==
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* Institute of Pathology, Fudan University, Shanghai, 200032, China.<ref name="ref2" />
 
* Institute of Pathology, Fudan University, Shanghai, 200032, China.<ref name="ref2" />
 
* Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.<ref name="ref2" />
 
* Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.<ref name="ref2" />
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* Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, China.<ref name="ref3" />
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* Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL, USA.<ref name="ref4" />
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* Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, PR China.<ref name="ref4" />
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* Cancer Institute, University of Mississippi Medical Center, Jackson, MS, USA.<ref name="ref4" />
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* Department of Endocrinology, PLA General Hospital, Beijing 100853, PR China.<ref name="ref4" />
  
 
==References==
 
==References==
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<ref name="ref2"> Qi P, Xu MD, Ni SJ, Huang D, Wei P, Tan C et al. Low expression of LOC285194 is associated with poor prognosis in colorectal cancer[J]. Journal of translational medicine. 2013, 11(1):122.
 
<ref name="ref2"> Qi P, Xu MD, Ni SJ, Huang D, Wei P, Tan C et al. Low expression of LOC285194 is associated with poor prognosis in colorectal cancer[J]. Journal of translational medicine. 2013, 11(1):122.
 
</ref>(2)
 
</ref>(2)
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<ref name="ref3"> Xu J, Zhao J, Zhang R. The novel long noncoding RNA TUSC7 inhibits proliferation by sponging MiR-211 in colorectal cancer[J]. Cellular physiology and biochemistry. 2017, 41(2):635-644.
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</ref>(3)
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<ref name="ref4"> Liu Q, Huang J, Zhou N, Zhang Z, Zhang A, Lu Z et al. LncRNA loc285194 is a p53-regulated tumor suppressor[J]. Nucleic acids research. 2013, 41(9): 4976-4987.
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</ref>(4)
 
</references>
 
</references>
  

Latest revision as of 08:32, 10 August 2019

TUSC7 is a p53-regulated tumor suppressor long noncoding gene, which acts in part through repression of miR-211

Annotated Information

Name

TUSC7: Tumor suppressor candidate 7 (HGNC nomenclature), also known as LOC285194, LSAMP antisense RNA 3 [1][2]. Approved symbol: RN7SK

Approved name: TUSC7

HGNC ID: HGNC:27701

Previous names: non-protein coding RNA 295; LSAMP antisense RNA 3; tumor suppressor candidate 7 (non-protein coding)

Previous symbols: NCRNA00295; LSAMP-AS3

Alias symbols: LINC00902; LOC285194

RefSeq ID: NR_015391

LncBook ID: HSALNT0059175


Characteristics

TUSC7 is four exon transcript of 2105 bp long noncoding RNA, located on the focal region of human chromosome chr3q13.31 (osteo3q13.31) [1][2]. Exon 4 harbors two miR-211 binding sites [2].

Function

Together with the other noncoding RNA BC040587 and the tumor suppressor gene LSAMP (limbic system-associated membrane protein) present in focal region (osteo3q13.31), TUSC7 cooperatively acting as tumor suppressor genes [1].

Depletion of TUSC7 by siRNA promoted proliferation of normal osteoblasts (with a mild increase in the G1 population) by cell-cycle transcripts (Cyclin D1, VEGF, and VEGFR1 up-regulation, and cyclin A2 and cyclin B1 suppression) as well as regulation of apoptotic genes (such as suppression of BCL2 and BimEL pro-apoptotic genes) [1].

Expression

TUSC7 is significantly decreased in Colorectal cancer tissues [3][4].

TUSC7 is downregulated in osteosarcoma [1].

Experiment Forward primer Reverse primer
qRT-PCR 5’-GGAAACAGAAGGCACCTCA-3’ 5’-TCTCAGAGGTCAAACAGGCA-3’[4]

Regulation

p53 transcriptionally regulates the TUSC7 by direct binding to its promoter region [2].

Disease

Labs working on this lncRNA

  • Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.[3]
  • Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.[3]
  • Institute of Pathology, Fudan University, Shanghai, 200032, China.[3]
  • Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.[3]
  • Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, China.[4]
  • Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL, USA.[2]
  • Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, PR China.[2]
  • Cancer Institute, University of Mississippi Medical Center, Jackson, MS, USA.[2]
  • Department of Endocrinology, PLA General Hospital, Beijing 100853, PR China.[2]

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Pasic I, Shlien A, Durbin AD, Stavropoulos DJ, Baskin B, Ray PN et al. Recurrent focal copy-number changes and loss of heterozygosity implicate two noncoding RNAs and one tumor suppressor gene at chromosome 3q13. 31 in osteosarcoma[J]. Cancer research. 2010, 70(1): 160-171.
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 Liu Q, Huang J, Zhou N, Zhang Z, Zhang A, Lu Z et al. LncRNA loc285194 is a p53-regulated tumor suppressor[J]. Nucleic acids research. 2013, 41(9): 4976-4987.
  3. 3.0 3.1 3.2 3.3 3.4 3.5 Qi P, Xu MD, Ni SJ, Huang D, Wei P, Tan C et al. Low expression of LOC285194 is associated with poor prognosis in colorectal cancer[J]. Journal of translational medicine. 2013, 11(1):122.
  4. 4.0 4.1 4.2 4.3 Xu J, Zhao J, Zhang R. The novel long noncoding RNA TUSC7 inhibits proliferation by sponging MiR-211 in colorectal cancer[J]. Cellular physiology and biochemistry. 2017, 41(2):635-644.

Sequence

>gi|285194|ref|NR_015391.1| Homo sapiens tumor suppressor candidate 7 (TUSC7), long non-coding RNA

000001 GGGGTACCAA AGTCCACTCT GAGCTTTTCC TCTGGGAACA GATCCCAGAT TATTCCAGAC TTAAGACATG GAGATATATC 000080
000081 TTGCTAGAGG TAGGAAGATG ATAAGATCAG TTTCTATAGG TAGAGGATAA TGTGTCCTAA AATGTAGGTA GAGATAAGTC 000160
000161 TGACAGTGGT TTAGACAAAA AGGGCCTCTG CTAATGGTGG AGAGATCAAG GAAGAAAAGA GCAACGTTAA AGTGAGGCTG 000240
000241 TACCCACAGA TCATGCTCAA GCAAATTCGA ACCGTGAGCG CATTTCTCTT AAACAATGAA CACTTAGAAT AAAAATTAAA 000320
000321 ATTTCCCACA AATTTCTAAT TTTAAAATAG TTTCCCAAGA TTTCAGTGGT ATTGTATATA TGCCCATATA ATTTTTTGGA 000400
000401 TTACTATTCC CACAAGTGTA AGATGAAAGA GGACCTGCCC TCCATTCTAT CTACTCCCTC TCTGCAAAGG CCATTTTGAA 000480
000481 GATCTCAGTT TTACTGAAAG GGGTCTGCCA CACATAACTT CCCTGGTGTA CCAGCAACAA GTGTTCAGTG GACCATGGCA 000560
000561 ACACAAAGAA TGACGGAGGA GAGAAAATGC CATGCAGAAG TGATAGTCAA GATGAAGATG TGGGAGAGGA GCAGCCCCCA 000640
000641 GTATATATTC AGTGTGTCCT CTGAAGTAAC AACCATCCCA TCTGGCTGAG AAGTGATGGA CACGGCTCCT TAACCACACT 000720
000721 GTACCCTCTG TTCTTGGCAC CTCTCTACCA GAAAGATATC AACAAATTGG ATGGAATTCA GAGAACAGTA AGAAAAATGA 000800
000801 TTAAAGAGAT GGAGGCAGTG ACTTATGAGG AAAGATGAAA AGGACTAAAT CTATGTAGCT TGGCAAAGCA ACAACTGAGG 000880
000881 TGGCGTATGA AAACTGTCTA CAAGCATTTG AAGAATATAA ACACCAAGGG AAGAGAGGGA ACTTTTTAGA ATGGTCTGGG 000960
000961 GAATAAGCAG AGTAAGAGGA CAGAATTGAA GAAAGGAGAG AGATATGCTA AGTTGGGGGT GGGGGCGTTT TCTCATCAAT 001040
001041 TGTAAATTTT GATAAATAAC AAAATTAAGT CTGGAGAGAA AGAGTTGCAT CTCATGCTTT TAGGTTACAA TCAGTGTTGA 001120
001121 CCTGCACTGA GAAAATGTGA CTTCCTATTA TCCTTCTCAA AGTTATTGCT GCAGAGGAAA GAAGCATACA TCTTTTACCC 001200
001201 ACCAGGAAAC CCCCAAAGCA TCTATTACCA TAATAGCCAT GGGAAACAGA AGGCACCTCA AATAAAGGTG GGGAAAAGAA 001280
001281 TGAAAGAAAT GGCTTTGGCC TGTGCCTGTT TGACCTCTGA GAGATACTTT TTGCAAGAAA TTGTTAAGTT TTGGCCCAAA 001360
001361 AAGTGGTC