NONHSAT080206

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Annotated Information

Name

Zfas1: Zinc finger antisense 1

Characteristics

Expressed from a bi-directional promoter with the protein-coding gene Znfx1. Zfas1 5°Ø is antisense to 5°Ø of Znfx1. Spliced, polyadenylated. SnoRNA host gene, contains 3 intronic C/D box snoRNAs. Human orthologue is alternatively spliced.

Disease

breast cancer

Function

Zfas1 appears to have a role in mammary gland proliferation and differentiation. - Zfas1 knockdown in a HC11 cell culture model of mammary differentiation showed increased differentiation and a probable increase in proliferation. Knockdown had little effect on intronic snoRNA expression suggesting the Zfas1 host transcript is functional. - Zfas1 knockdown appears to affect Znfx1 expression.

Expression

Highly expressed and differentially expressed during mammary development. Strongly down-regulated during lactation compared to pregnancy and then up-regulated again during involution. Znfx1 coding gene,in contrast, was more lowly expressed and was not differentially expressed during mammary gland development. Expressed in the epithelial cells of the mammary gland ducts and alveoli. Zfas1 is widely expressed in mouse tissues including brain, liver, lung, kidney, spleen, heart and embryo, showing higher expression than Znfx1. Zfas1 extremely stable with a half life of >32 hrs in N2A (neuroblastoma) cells. Compared to a half life of 50 minutes for Znfx1. The increased stability of Zfas1 may explain why its expression level is much higher than Znfx1. However, in mouse 3T3 and embryonic stem cells Zfas1 stability was much lower with a half-life of 1.7 hr and 63 min respectively, while the human ZFAS1 had a half-life of 3 hr in B-cells (Friedel (2009), Sharova (2009), Clark (2012)). These results suggest there is significant regulation of Zfas1 stability both within and between species (Clark (2012)). Localised to both the nucleus and the cytoplasm. Human ZFAS1 expression is reduced in ductal carinoma compared to normal tissue.

Conservation

Syntenic transcription observed in multiple mammals. Low conservation of Zfas1 sequence between human and mouse compared to snoRNAs, but Zfas1 has potential secondary structural similarities.

Misc

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Transcriptomic Nomeclature

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Regulation

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Allelic Information and Variation

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Evolution

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Labs working on this lncRNA

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References

Annotation originally sourced from lncRNAdb.

Basic Information

Transcript ID

NONHSAT080206

Source

NONCODE4.0

Same with

,

Classification

intergenic

Length

1020 nt

Genomic location

chr20+:47894715..47905797

Exon number

5

Exons

47894715..47895253,47895641..47895745,47897022..47897107,47897440..47897501,47905582..47905797

Genome context

Sequence
000001 CGGGGGCCCA GGGTGGAGAG CACGAGGGCC TGGCCCAGGC ACGGCCGGCG CCTCCGCCCT CGAGGAGGGC GTCACCTCAG 000080
000081 CTCCCCCCGG CGGCGGAGCC GGCGGGCTCA GGCGGGCGCG GCTGAGGGGA GCGGACCGCG GGGGGCGGGA GATGACTGCG 000160
000161 CCCAAGGCCT TTGCGGGCCT CAGCCGGCCC CAGAGGAAGG GGAACCCGTC GAGCGGTTTG GTGCGTGTGA AGCGCGACAT 000240
000241 GGCGAGGAAG CGGACAAGCC CGGGTGGCCC GGCGTGTAGA GGGAAGGGGG CGGGGCTAGA CGCGGCCTGG ACAACTACTA 000320
000321 GAGCGCCTCG GGCTGTGCTG CTCGAGACTA CATTTCCCAG AGCGACGCGC GCGGAGCGGG CGGGAAAGAG AGCGTTTCGG 000400
000401 GTCCAGTGCG CAGGTGCGAA AGCCATCTTT GGTTATATAA GGGAGGTTCA GGAAGCCATT CGTTCTTTCG CGTCTGCGGT 000480
000481 GCCCGGAGTG TGGTACTTCT CCTAGTTGCA GTCAGGCTTC ATACGCTATT GTCCTGCCCG TTAGAGCAGC CAGCGGGTAC 000560
000561 AGAATGGATT TTGGAAGAGG GAGTCACCAC TGGACCTCCA AGGAAGCCAC GTGCAGACAT CTACAACCTT CGATCTCCTG 000640
000641 ACGAGTTTAT TGTTGGCCAA AACCAGGCTT TGATTGAACC AGGATGAATG CGGGTGTTGG AAGTAGAATA TATATATACA 000720
000721 TATAAAATTG GTTGGGAGCC ACGTGTACCA GTGTGTGTTG ATCTTGGCTT GATTCAGTCT GCCTTGTAAC AGAAACTGGC 000800
000801 GATGGAATAT GAGAGGAGCC CTCTGGAAAG AAAAGGACAG ACCCTGTGCT TTCATGAAAG TGAAGATCTG GCTGAACCAG 000880
000881 TTCCACAAGG TTACTGTATA CATAGCCTGA GTTTAAAAGG CTGTGCCCAC TTCAAGAATG TCATTGTTAG ACTTTGAAAT 000960
000961 TTCTAACTGC CTACCTGCAT AAAGAAAATA AAATCTTTTA AATCAAAAAA AAAAAAAAAA
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