Difference between revisions of "LINC01672"

From LncRNAWiki
Jump to: navigation, search
(Created page with "''LINC01672'', long intergenic non-protein coding RNA 1672, an important key regulator of esophageal squamous cell carcinoma (ESCC) tumor metastasis and drug resistance. ==A...")
 
 
Line 8: Line 8:
  
 
===Characteristics===
 
===Characteristics===
5′ and 3′ RACE analyses were performed to determine the full length of NMR. 5′ and 3′ RACE analyses were performed to determine the full length of NMR. The full-length NMR was then validated by Northern blot. Northern blot identified strong bands around 1800 nt which was consistent with RACE results and NMR was overexpressed in tumor tissues. Cellular localization provides important information about potential functions of lncRNAs. RNA FISH and subcellular fractionation assays showed that most of NMR located in the cell nucleus rather than cytoplasm, red fluorescence which indicated NMR could only be seen in nucleus and over 95% of NMR was detected in nucleic RNAs.  
+
5′ and 3′ RACE analyses were performed to determine the full length of NMR. 5′ and 3′ RACE analyses were performed to determine the full length of NMR. The full-length NMR was then validated by Northern blot. Northern blot identified strong bands around 1800 nt which was consistent with RACE results and NMR was overexpressed in tumor tissues. Cellular localization provides important information about potential functions of lncRNAs. RNA FISH and subcellular fractionation assays showed that most of NMR located in the cell nucleus rather than cytoplasm, red fluorescence which indicated NMR could only be seen in nucleus and over 95% of NMR was detected in nucleic RNAs. <ref name="ref1" />
  
 
===Function===
 
===Function===
Functionally, NMR could promote tumor cell migration and invasion, inhibit cisplatin-induced apoptosis and increase drug resistance in ESCC cells. NMR could directly bind to chromatin regulator BPTF, and potentially promote MMP3 and MMP10 expression by ERK1/2 pathway through recruiting BPTF to chromatin. Taken together, NMR functions as an oncogenic gene and may serve as new biomarker and therapeutic target in ESCC.
+
Functionally, NMR could promote tumor cell migration and invasion, inhibit cisplatin-induced apoptosis and increase drug resistance in ESCC cells. NMR could directly bind to chromatin regulator BPTF, and potentially promote MMP3 and MMP10 expression by ERK1/2 pathway through recruiting BPTF to chromatin. Taken together, NMR functions as an oncogenic gene and may serve as new biomarker and therapeutic target in ESCC.<ref name="ref1" />
  
 
===Regulation===
 
===Regulation===
Transcription of NMR could be upregulated by NF-κB activation after IL-1β and TNF-α treatment. NMR was methylated by NSUN2 and might competitively inhibit methylation of potential mRNAs.
+
Transcription of NMR could be upregulated by NF-κB activation after IL-1β and TNF-α treatment. NMR was methylated by NSUN2 and might competitively inhibit methylation of potential mRNAs.<ref name="ref1" />
  
 
===Expression===
 
===Expression===
LncRNA NMR is overexpressed in ESCC tissues. Lymph node positive HNSC patients had significantly higher NMR expression; meanwhile, overexpression of NMR was also significantly associated with distal tumor metastasis in  head and neck squamous cell carcinoma (HNSC) patients.  
+
LncRNA NMR is overexpressed in ESCC tissues. Lymph node positive HNSC patients had significantly higher NMR expression; meanwhile, overexpression of NMR was also significantly associated with distal tumor metastasis in  head and neck squamous cell carcinoma (HNSC) patients. <ref name="ref1" />
  
 
===Diseases===
 
===Diseases===
* Esophageal squamous cell carcinoma (ESCC)
+
* Esophageal squamous cell carcinoma (ESCC) <ref name="ref1" />
* Head and neck squamous cell carcinoma (HNSC)
+
* Head and neck squamous cell carcinoma (HNSC) <ref name="ref1" />
  
 
==Labs working on this lncRNA==
 
==Labs working on this lncRNA==

Latest revision as of 07:09, 19 December 2018

LINC01672, long intergenic non-protein coding RNA 1672, an important key regulator of esophageal squamous cell carcinoma (ESCC) tumor metastasis and drug resistance.

Annotated Information

Name

LINC01672: long intergenic non-protein coding RNA 1672 (HGNC:52460)

Aliases

NMR: NSUN2 methylated lncRNA

Characteristics

5′ and 3′ RACE analyses were performed to determine the full length of NMR. 5′ and 3′ RACE analyses were performed to determine the full length of NMR. The full-length NMR was then validated by Northern blot. Northern blot identified strong bands around 1800 nt which was consistent with RACE results and NMR was overexpressed in tumor tissues. Cellular localization provides important information about potential functions of lncRNAs. RNA FISH and subcellular fractionation assays showed that most of NMR located in the cell nucleus rather than cytoplasm, red fluorescence which indicated NMR could only be seen in nucleus and over 95% of NMR was detected in nucleic RNAs. [1]

Function

Functionally, NMR could promote tumor cell migration and invasion, inhibit cisplatin-induced apoptosis and increase drug resistance in ESCC cells. NMR could directly bind to chromatin regulator BPTF, and potentially promote MMP3 and MMP10 expression by ERK1/2 pathway through recruiting BPTF to chromatin. Taken together, NMR functions as an oncogenic gene and may serve as new biomarker and therapeutic target in ESCC.[1]

Regulation

Transcription of NMR could be upregulated by NF-κB activation after IL-1β and TNF-α treatment. NMR was methylated by NSUN2 and might competitively inhibit methylation of potential mRNAs.[1]

Expression

LncRNA NMR is overexpressed in ESCC tissues. Lymph node positive HNSC patients had significantly higher NMR expression; meanwhile, overexpression of NMR was also significantly associated with distal tumor metastasis in head and neck squamous cell carcinoma (HNSC) patients. [1]

Diseases

  • Esophageal squamous cell carcinoma (ESCC) [1]
  • Head and neck squamous cell carcinoma (HNSC) [1]

Labs working on this lncRNA

  • Department of Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China
  • Central Laboratory, Beijing Luhe Hospital, Capital Medical University, Beijing, China
  • Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, China
  • Tumor Hospital of Shanxi Province, Taiyuan, Shanxi, 030013, China

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Li Y, Li J, Luo M, Zhou C, Shi X, Yang W et al. Novel long noncoding RNA NMR promotes tumor progression via NSUN2 and BPTF in esophageal squamous cell carcinoma[J]. Cancer letters. 2018, 430:57-66.

Sequence

>gi|100505887|ref|NR_104620.1| Homo sapiens long intergenic non-protein coding RNA 1672 (LINC01672), transcript variant 1, long non-coding RNA

000001 GCCTGACTCA TGAAGCGTGG AGCGACCCAG GAACATCCCA GCTCCTCATG TCCCACATTC TTCAGGCTTT CTCTTAGTTT 000080
000081 TGGCATGTGC ATGTAAATGT CAACAGATCA ATAAAGGCAA AAACCAGGAG ATCCCATAAA TCCCGCTGAG CCTGTCATAG 000160
000161 CTATCAAATT ATACCCAGAT AAAGCTTCTG GTGGCTAATG ACATGGCCAT CACCCAGCGT GGACGTGGGC AGGAGGGGCT 000240
000241 CTCCGGGAGC ATCTGGTCTG CTGTGGAGAC ACTTGTCATC TGGAGGCTCT GTGGCTCTAA TGGAGGGTGA TGCTTTGAAC 000320
000321 GCGACTATCT CCCCCATAAA AAAATAAATA AATAATAAGT ATAATTTAAA AATAAACAAA GATCTGGATT TGCAGGCTAA 000400
000401 AAAAAAAAAA A