Difference between revisions of "LINC01672"
(Created page with "''LINC01672'', long intergenic non-protein coding RNA 1672, an important key regulator of esophageal squamous cell carcinoma (ESCC) tumor metastasis and drug resistance. ==A...")
Revision as of 07:07, 19 December 2018
LINC01672, long intergenic non-protein coding RNA 1672, an important key regulator of esophageal squamous cell carcinoma (ESCC) tumor metastasis and drug resistance.
LINC01672: long intergenic non-protein coding RNA 1672 (HGNC:52460)
NMR: NSUN2 methylated lncRNA
5′ and 3′ RACE analyses were performed to determine the full length of NMR. 5′ and 3′ RACE analyses were performed to determine the full length of NMR. The full-length NMR was then validated by Northern blot. Northern blot identified strong bands around 1800 nt which was consistent with RACE results and NMR was overexpressed in tumor tissues. Cellular localization provides important information about potential functions of lncRNAs. RNA FISH and subcellular fractionation assays showed that most of NMR located in the cell nucleus rather than cytoplasm, red fluorescence which indicated NMR could only be seen in nucleus and over 95% of NMR was detected in nucleic RNAs.
Functionally, NMR could promote tumor cell migration and invasion, inhibit cisplatin-induced apoptosis and increase drug resistance in ESCC cells. NMR could directly bind to chromatin regulator BPTF, and potentially promote MMP3 and MMP10 expression by ERK1/2 pathway through recruiting BPTF to chromatin. Taken together, NMR functions as an oncogenic gene and may serve as new biomarker and therapeutic target in ESCC.
Transcription of NMR could be upregulated by NF-κB activation after IL-1β and TNF-α treatment. NMR was methylated by NSUN2 and might competitively inhibit methylation of potential mRNAs.
LncRNA NMR is overexpressed in ESCC tissues. Lymph node positive HNSC patients had significantly higher NMR expression; meanwhile, overexpression of NMR was also significantly associated with distal tumor metastasis in head and neck squamous cell carcinoma (HNSC) patients.
- Esophageal squamous cell carcinoma (ESCC)
- Head and neck squamous cell carcinoma (HNSC)
Labs working on this lncRNA
- Department of Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China
- Central Laboratory, Beijing Luhe Hospital, Capital Medical University, Beijing, China
- Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, China
- Tumor Hospital of Shanxi Province, Taiyuan, Shanxi, 030013, China
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>gi|100505887|ref|NR_104620.1| Homo sapiens long intergenic non-protein coding RNA 1672 (LINC01672), transcript variant 1, long non-coding RNA