HAS2-AS1
Contents
Annotated Information
Name
HAS2-AS1：HAS2 antisense RNA 1
HASNT, NCRNA00077[1]
Characteristics
Natural antisense mRNAs of human and mouse HAS2 that are transcribed from the opposite strand of the HAS2 gene locus. Many of these clones are oriented in a direction consistent with their transcription from the opposite strand of the HAS2 locus, representing putative cis-encoded natural antisense mRNAs of HAS2. [1]
Function
Expression of L-HASNTs or S-HASNTs strongly reduces HAS2 mRNA levels. L-HASNT-expressing clones has an inhibition of the HAS2 mRNA level ranging from 56 to 94%, and S-HASNT-expressing clones has an inhibition of the HAS2 mRNA level from 46 to 86%, compared with the clones transfected with vector only. Expression of HASNTs also inhibited HA biosynthesis. In addition, cell growth is noticeably inhibited in the L- and S-HASNT-expressing clones.[1]
Expression
HASNT is expressed at higher levels in skin, prostate gland, uterus, and salivary gland tissues. Furthermore, both splice variants of many cell lines could be detected by RT-PCR, including HuT12 (human tumor cell line), WI38 (human embryonic lung fibroblast), and primary human dermal fibroblasts, along with mouse 3T6 (em- bryonic fibroblast) cells (data not shown). [1]
Sequence
>gi|299829247|ref|NR_002835.2| Homo sapiens HAS2 antisense RNA 1 (HAS2-AS1), antisense RNA
000081 CGTTCCCTCC CGTAGCTGGG CTGCTGCAGC TCGTTCGCCC CAGAGGCGCG CGGTGTCCTT GAGTCCAAGT ATTCAGCGCG 000160
000161 GACGTTTGGG GGACGGAGGG AGAGGCGCTA GGGGAACTGG CACCTAAGAC TGTGTAATAG GACACAGGTC TTGCAAGTCC 000240
000241 TCCTCCCCAG GCACCGCCCC TGTGCTCCCA GCGCCTCTTT GCAGCTTTAA TTATCCTTTC AGCGCCGTTC CCCCGCGGCT 000320
000321 GCGTTTCCGT GGTCAGAGGA AAAAGGCTCT TTCGCCTCCC GGTTCTCACC CTCATACAGA CCCTCCTTTC AGACCCGCCC 000400
000401 TCCTGTTCAC AGGGACCCAT TTCGGAGCCC TGGCTCACGC TGGGCGGGAA CTGCCGTGAC GAATTCCCCC CAAGGTGTTG 000480
000481 ATTATCCATC CCAACCCAGC CTCTGGCTTC CCTCCCACCT CCACCCCAGC TCCCACAGGC CCCTCGCCCC GTTCATCGGC 000560
000561 TGGGCGCTCG AGACGGCGCC CCCAGATCTC CCACCGGGCA GTGTCCCCAG CTGCAGCTGA GGAGAGAAGC GCCGGCGTGC 000640
000641 GCGGTGCCAC GTCGGAATTG GCTCTGGGGA CGCCGCAGCT GCCGAAGCGC AGCGCGCGGT CCCCACGCAC GCCCTGCCTC 000720
000721 CGGGCGGCCG GCGGGCTGGC CCGGCGAGGA GAGGAGGGAT GCGCGCCCTC CTCCCGCTCC TATCTGCGGG TGCCACCGCC 000800
000801 TGCGTCAGGG ACCCCTGGCC CGATCTTTCT GCCCCCGATA ACAGAAAATC TCTTTTTCGT CTTAAAAAAA AAAAAAAAAA 000880
000881 AAAAAAAAGC CTGTGGAAGA CTCAGCAGAA CCCAGGAAGC GCAGAATTGG GAGAAAAGTC TTTGGCTGGG GCTGTTTCAA 000960
000961 GTCTCTGGTT CAATGGGCTG CTCGAAGCCA GGACTGGGTA ATTCTTTCCA GACGTCTTGA CTTCTCCTTC CCCGCCGTTG 001040
001041 TTGCCCTTCT GCCTCCTCCA ACTTAAGGGG GTCTTAACAA GATCTTCTGA GGGGTGGACT TCTTTGGAAC TCGACAGGAC 001120
001121 CTTGAAGACT GGGTTCTTAG TCCTTATCAC ATATATGGGC TATAATGAAT AAGTCTAAAC AGAAAAGTCG AATAAACTGG 001200
001201 AAATGATGCT TTCATCACGT CGCACAAGGA GCTGTTTGGA TCAGAAGATG ACATCCTTTC ACCCATCCCT TTCAGGTTTT 001280
001281 GACATCCTAA AATGTACCTT ATGGAACCCA TACCTGTTAT GTTTTCACCT GTACAAGGCA ACAGAGAAAA CATGGTAGTT 001360
001361 TTTACTTGAG TAGATGTGAC TTAGCAATAT GTTGTTGCTG ACCTCCATCC CACAGCACAA AACATATGTT TATATGTCTG 001440
001441 ACAGACAAAG TATCCAGAAC AAGGATTTAG TACCTTCATA ATCCTCTGTA TATGTGGTTC TAGAACTTTT GGTAAAGAAA 001520
001521 CTCTTTATAC TCTTATTAAT TATTGAGAAC CTCAAAAAAA GTTTATATGT TATATGTATC AATATTTACC ACATTAGACA 001600
001601 TTAAAGCTGA CTTAAAAAGT TAAAATACTT AAGTCATTTG AAGTAATAAT AAATGCAGTT ACATACCAAC TTAATAACAT 001680
001681 TTTGCGAAAA TAGCTATTTT TCAAAACCTA AAGTGAGAAA AGTGGCAGTT TTCCATTTTT GCAAATCTCT TTGCTGTCTA 001760
001761 GCTGATAGAA GACAGCTGGA TTCTCCTATT TGCTTTTGCA TTCAATTGTT GTTTTGATTG AAGTATATGA AAAAAAATCT 001840
001841 GGTGTCATAC ACATATATAA TTGGGAAATG GAAGAGTATT TACACAGGCT TTTTTAGATA TTCTTCCTTT ATACTACATC 001920
001921 AAAACTTGAT AAGTTGCAAT GTTGTGAATT GGCCCAGGGC TTGGCCCTCT GAAGATTTGC TGAAAATCAC TGACATGAAG 002000
002001 TAGATTAATA GGATAAAAGG CATATACATT TATTTAATGT GTATACACAG CGGCCTTTAG AATGAAGACC CAAAGATACA 002080
002081 GAAGAAATTG TCTGTTTTTA TGCTTAAGTT TAACAAAGAA TGGGCAGCCG TGTAGAGATA GGATAGGACA AAAAGGGCAT 002160
002161 AATCTAATGC TAGCTGACTG AGTGAGGAAG CCCAGCAAGG CCTGTCAGTG TCTCCTCAGT ATGAGTGAAT TTTGTGTACT 002240
002241 CTGATAATAC ATTAAAATCT GTTTATGTAC TCTGATAACA CATTCAAAAA AAAAAAAAAA
Labs working on this lncRNA
- Center for Extracellular Matrix Biology, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, Texas 77030, USA.
