Difference between revisions of "ENST00000517411.1"
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===Function=== | ===Function=== | ||
MINCR could interplay with transcriptor MYC to co-regulate the expression level of a group of genes involved in cell cycle initiation and progression <ref name="ref1" />. | MINCR could interplay with transcriptor MYC to co-regulate the expression level of a group of genes involved in cell cycle initiation and progression <ref name="ref1" />. | ||
| + | |||
| + | ===Disease=== | ||
| + | Burkitt lymphoma | ||
===Regulation=== | ===Regulation=== | ||
| Line 16: | Line 19: | ||
===Expression=== | ===Expression=== | ||
| + | [[File:F6.large.jpg|right|thumb|200px|'''Correlation between MINCR and MYC expression in cancer.'''<ref name="ref1" />.]] | ||
| + | |||
MINCR strongly correlates with MYC expression in not only BL but more in general, GC-derived MYC-positive lymohomas and other cancer types, like pancreastic ductal adenocarcinomas, that are known to rely on MYC expression<ref name="ref1" />. | MINCR strongly correlates with MYC expression in not only BL but more in general, GC-derived MYC-positive lymohomas and other cancer types, like pancreastic ductal adenocarcinomas, that are known to rely on MYC expression<ref name="ref1" />. | ||
| − | === | + | {| class='wikitable' style="text-align:center" |
| − | + | |- | |
| + | ! | Primer | ||
| + | ! | Forward primer | ||
| + | ! | Reverse primer | ||
| + | |- | ||
| + | | rowspan="1"|qRT-PCR | ||
| + | | | 5'-TGTGGCAAAACTTGAATGGA-3' | ||
| + | | | 5'-GGGGGAGGACAAGAGAAAGA-3'<ref name="ref1" /> | ||
| + | |- | ||
| + | | rowspan="1"|siMINCR_1 | ||
| + | | | 5'-GGGAAGAGUGCGUCUGUGAUU-3' | ||
| + | | | 5'-UCACAGACGCACUCUUCCCUU-3'<ref name="ref1" /> | ||
| + | |- | ||
| + | | rowspan="1"|siMINCR_2 | ||
| + | | | 5'-GAGCCUUGUUUGCCAUUAAUU-3' | ||
| + | | | 5'-UUAAUGGCAAACAAGGCUCUU-3'<ref name="ref1" /> | ||
| + | |} | ||
===Evolution=== | ===Evolution=== | ||
| − | + | MINCR is conserved throughout primates, with the exception of Galago, but not in other vertebrates <ref name="ref1" />. | |
| − | |||
| − | |||
==Labs working on this lncRNA== | ==Labs working on this lncRNA== | ||
| − | + | * Fred Hutchinson Cancer Research Center <ref name="ref1" /> | |
==References== | ==References== | ||
| − | + | <references> | |
| + | <ref name="ref1"> Doose G, Haake A, Bernhart SH, Lopez C, Duggimpudi S, Wojciech F, et al. MINCR is a MYC-induced lncRNA able to modulate MYC's transcriptional network in Burkitt lymphoma cells[J]. Proceedings of the National Academy of Sciences of the United States of America. 2015,112(38):E5261-70.</ref>(1) | ||
| + | </references> | ||
{{basic| | {{basic| | ||
tID = ENST00000517411.1| | tID = ENST00000517411.1| | ||
source = Gencode19| | source = Gencode19| | ||
| − | same = lnc-TOP1MT-2:5| | + | same = lnc-TOP1MT-2:5|MINCR |
classification = intergenic| | classification = intergenic| | ||
length = 645 nt| | length = 645 nt| | ||
Latest revision as of 12:21, 1 July 2016
MINCR, short for MYC-induced long noncoding RNA, is a novel player in MYC transcriptional network,with the potential to open new therapeutic windows in the fight against maglignant lymphoma and, possibly, all cancers that rely on MYC expression.
Contents
Annotated Information
Transcriptomic Nomeclature
MINCR: MYC-induced long noncoding RNA [1] LINC01604, "long intergenic non-protein coding RNA 1604"[1]
Characteristics
The MINCR gene, which is located on chromosome 8q24.3 and intergenic to the two coding genes GLI4 and ZNF696, has a long transform composed of three exons. It is ubiquitously expressed, with the highest expression values in brain, prostate, and testis [1].
Function
MINCR could interplay with transcriptor MYC to co-regulate the expression level of a group of genes involved in cell cycle initiation and progression [1].
Disease
Burkitt lymphoma
Regulation
MINCR is found to be induced by MYC in not only restricted to B cell line but in MYC-regulated retinal pigment epithelial cell line hT-RPE-MycER [1].
Expression
MINCR strongly correlates with MYC expression in not only BL but more in general, GC-derived MYC-positive lymohomas and other cancer types, like pancreastic ductal adenocarcinomas, that are known to rely on MYC expression[1].
| Primer | Forward primer | Reverse primer |
|---|---|---|
| qRT-PCR | 5'-TGTGGCAAAACTTGAATGGA-3' | 5'-GGGGGAGGACAAGAGAAAGA-3'[1] |
| siMINCR_1 | 5'-GGGAAGAGUGCGUCUGUGAUU-3' | 5'-UCACAGACGCACUCUUCCCUU-3'[1] |
| siMINCR_2 | 5'-GAGCCUUGUUUGCCAUUAAUU-3' | 5'-UUAAUGGCAAACAAGGCUCUU-3'[1] |
Evolution
MINCR is conserved throughout primates, with the exception of Galago, but not in other vertebrates [1].
Labs working on this lncRNA
- Fred Hutchinson Cancer Research Center [1]
References
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Doose G, Haake A, Bernhart SH, Lopez C, Duggimpudi S, Wojciech F, et al. MINCR is a MYC-induced lncRNA able to modulate MYC's transcriptional network in Burkitt lymphoma cells[J]. Proceedings of the National Academy of Sciences of the United States of America. 2015,112(38):E5261-70.
Basic Information
| Transcript ID |
ENST00000517411.1 |
| Source |
Gencode19 |
| Same with |
lnc-TOP1MT-2:5 |
| Classification |
{{{classification}}} |
| Length |
645 nt |
| Genomic location |
chr8-:144362336..144363830 |
| Exon number |
3 |
| Exons |
144362336..144362685,144363052..144363167,144363652..144363830 |
| Genome context |
|
| Sequence |
000001 TCGCGTGTCT TCCGAACTCT GCTGCTTCGC CCCGGCAAGA GCACTTCCTT CCGCGGCTGC TAAAACTGGT GCGCGGGTTC 000080
000081 CCAACTTCGC AGAAGAGCTT CATCGGCCCC CCGCTAGAGA CCTCAGACCG CAGCCATCGA CGCCTCGCCT CGGGGATCTG 000160 000161 TCCCCACCCT CGGGGCCAGC TCACCATGAG TCAGGCCCTG TGCTAGGTGC TGGGATGAAG CAGTGACTGA CACAGGCCTG 000240 000241 GACCTGTCCT TGGGAAGAGT GCGTCTGTGA CACAGATACT GTGCCATGTG GCAAAACTTG AATGGAGACA TCAGCTCTTT 000320 000321 GTGGGTCTCA AGTCTACTGG CTTTCAATCA GCTCTTCTGC TTGACAGCTG CAGGTCTTGG GACTGCCCAG CCCCCATAAC 000400 000401 CCTGTGAGTG CATTCCTGAT AATAACCCTC TTTCTCTTGT CCTCCCCCAC CCCACATCAT TGGTTCTGTT TCTCTGGAGA 000480 000481 ACCATAATAC ATCATTTACT AATCAGGAAA TAGATTCAGA GACATTAATA ACTCATCCAA CCTCACAGTT AACTGTAGAG 000560 000561 TTGGGCTTGA AATTCCAAGG TCGATTTTCT TAGCCAATAC ACCATGCTAC ATATATGTAT GTAAAAATAA AAACGCTTGT 000640 000641 GAACT |
