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| − | ''CASC8''：cancer susceptibility candidate 8,located in 8q24.21 region, is suggested with cancer, such as colorectal cancer, prostate cancer, intestinal tumor，<ref name="ref1" /> gastric cancer<ref name="ref2" />
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| − | ==Annotated Information==
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| − | ===Name===
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| − | ''CASC8'':cancer susceptibility candidate 8(non-protein coding)
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| − |
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| − | ''CARLo-1'',LINC008600,”long intergenic non-protein coding RNA 860” <ref name="ref1" />
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| − |
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| − | ===Characteristics===
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| − | Seven lncRNAs are located in the 8q24.21 gene desert. The region including CARLo-1∼6 <ref name="ref1" />
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| − |
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| − | ===Function===
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| − | [[File: Function of CARLo-5 in cell proliferation through cell-cycle regulation..jpg|right|thumb|400px|'''Function of CARLo-5 in cell proliferation through cell-cycle regulation.''' <ref name="ref1" />.]]
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| − | Inhibition of CARLo-5 dramatically reduced the number of cells and colonies in vitro, indicating a role of CARLo-5 in cell transformation. CARLo-5 is not only overexpressed in tumors but also involved in tumorigenesis. <ref name="ref1" />
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| − | CASC8 may presumably play a key role in modulating the recruitment of general transcription factors on the promoter of its cognate gene, POU5F1B, which was found to be a putative cancer susceptibility gene. It is biologically plausible that allele of rs10505477 further alters the fine turning interactions between the CASC8 and POU5F1B. <ref name="ref2" />.
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| − |
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| − | ===Regulation===
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| − | CASC8 regulated by rs10505477 ,which was involved in clinical outcomes of gastric cancer, act as a cancer susceptibility candidate. <ref name="ref2" />
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| − |
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| − | ===Diseases===
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| − | * colorectal cancer, prostate cancer, intestinal tumor<ref name="ref1"/>,gastric cancer<ref name="ref2"/>
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| − |
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| − | ===Expression===
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| − | CARLo-5 is overexpressed in CRC and involved in oncogenic transformation and tumor incidence.<ref name="ref1" />
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| − |
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| − | {| class='wikitable' style="text-align:center"
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| − | |-
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| − | ! | Target lncRNA
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| − | ! | Use
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| − | ! | Sequence
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| − | |-
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| − | | rowspan="1"|CARLo-1 v1&3
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| − | | | 5′ RACE
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| − |
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| − | 5′ nested PCR and sequencing
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| − |
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| − | Internal-F and sequencing
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| − |
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| − | Internal-R and sequencing
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| − | 3′ RACE
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| − | 3′ nested PCR and sequencing
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| − |
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| − | | |GGCAGGTTGTGTGTCACGCCCTCCACT
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| − | ATGTTTCATTGGCTGAAGCTCAATTG
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| − | CCTCAGGAACACACATGGATGATTCT
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| − | AAAGAGTATGGAGGCTTGAACATGGC
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| − | AGTGGAGGGCGTGACACACAACCTGCC
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| − | GCCATGTTCAAGCCTCCATACTCTTT<ref name="ref1" />
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| − | |-
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| − | | rowspan="1"|CARLo-1 v2&4
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| − | | | 5′ RACE
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| − | 5′ nested PCR and sequencing
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| − | Internal-F and sequencing
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| − | Internal-R and sequencing
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| − | 3′ RACE
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| − | 3’nested PCR and sequencing
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| − |
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| − | | | GCTCCTGGTCCATGCCATTCATCAATTG
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| − | GCTGAGGACAGAAGGCATTGGTCT
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| − | GAGCATCCGAATAAATAAACATACAAATGC
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| − | ATTTTTCACCTGCTTCAGTGCCATTGT
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| − | GAGACCAATGCCTTCTGTCCTCAGC
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| − | AATTGATGAATGGCATGGACCAGG<ref name="ref1" />
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| − | |-
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| − | | rowspan="1"|CARLo-2
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| − | | | 5′ RACE
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| − |
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| − | 5′ nested PCR and sequencing
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| − | Internal-F and sequencing
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| − |
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| − | Internal-R and sequencing
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| − |
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| − | 3′ RACE
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| − |
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| − | 3′ nested PCR and sequencing
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| − |
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| − | | | CCTTGACTTGAGCCCAGGTCACCATGC
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| − | ACGTCTTCTCTTGGCTCCTCTGGAT
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| − | AAACTATTGATGCAGTGTCCAAGGTGG
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| − | ACATCAGTTAAAAGGCCAACAGGAACC
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| − | GCATGGTGACCTGGGCTCAAGTCAAGG
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| − | CTTATTTCTCTGCTGAACCATCACA<ref name="ref1" />
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| − | |-
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| − | | rowspan="1"|CARLo-3
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| − | | | CARLo-3
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| − | | | TGGGCCTAGTACTTGGAGACGGGGACTG
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| − | TTGGATGATAGTGAAGGTGAACGTCTCT
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| − | AACGAAGAGCATCACCATTTCCACCA
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| − | ACTCTCCTAATGGGCCTAGTACTTGGAGAC
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| − | AACGAAGAGCATCACCATTTCCACCACC
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| − | AGAGACGTTCACCTTCACTATCATCCAA<ref name="ref1" />
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| − | |-
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| − | | rowspan="1"|CARLo-4
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| − | | | 5′ RACE
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| − |
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| − | 5′ nested PCR and sequencing
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| − | Internal-F and sequencing
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| − |
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| − | Internal-R and sequencing
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| − | 3′ RACE
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| − |
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| − | 3′ nested PCR and sequencing
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| − |
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| − | | | GTGACAGACGGAGTGCTGATGCCTCTGG
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| − | AATCTCTTCACATTGTGGAGTTTCTCCTC
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| − | ATCTAGCAAATAGGCATCTGTCACAGAAAATAGA
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| − | ATCAGCCTAACCAGCACGCATAAACTAAAC
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| − | CCAGAGGCATCAGCACTCCGTCTGTCAC
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| − | TCTGTCACTATTGGCATCCTGTGACA<ref name="ref1" />
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| − | |-
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| − | | rowspan="1"|CARLo-5
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| − | | | 5′ RACE
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| − | 5′ nested PCR and sequencing
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| − | Internal-F and sequencing
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| − | Internal-R and sequencing
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| − | 3′ RACE
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| − | 3′ nested PCR and sequencing
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| − |
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| − | | | GGGTTGAGAAGGGGTGAAGGGACACTGG
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| − | AAGTGAAGCTGGGAAGTTTGCACTTG
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| − | GCCACAAATCAACAACAACAACAACAA
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| − | AGAGTGATGCCAAGGCTGTTATTGTCAA
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| − | CCAGTGTCCCTTCACCCCTTCTCAACCC<ref name="ref1" />
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| − | |-
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| − | | rowspan="1"|CARLo-6
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| − | | | 5′ RACE
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| − | 5′ nested PCR and sequencing
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| − | Internal-F and sequencing
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| − | Internal-R and sequencing
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| − | 3′ RACE
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| − | 3′ nested PCR and sequencing
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| − |
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| − | | | GGGTGCCATCACAGTCAGTTTGGACAGC
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| − | CTTGAATGCTATCAGGGAAGCTAGAGTG
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| − | CTTAGAATTGGAGTGCCTGGGTTAGAA
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| − | GCAGTCATTCAGGAATTGAAGCTGATA
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| − | GCTGTCCAAACTGACTGTGATGGCACCC
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| − | AGACAGACAGCGATGCCACATGTT<ref name="ref1" />
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| − | |}
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| − | ===Sequence===
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| − | >gi|211971085|ref|NR_024393.1| Homo sapiens cancer susceptibility candidate 8 (non-protein coding) (CASC8), transcript variant 2, long non-coding RNA
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| − | <dnaseq>TGAACCACCCCAGGCCCTTTGCACCTGCTATTCCCTCTCCTCAGCATGCTCTGCCCCAGACAATTGCAAT
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| − | GCTAACTTCTTACTTCCTTCAGGTTTCACTCAGAAATGACTTTCTCAGTGGGGTCTCCCATGACCATTTT
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| − | ATTTAAGATTTCAACTACTTTGCCAGTATTTCACTTCTCCTCCCTTGATCTTTTCTTCCAAGCACTCACT
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| − | GCCCTCTTGCACACTGTATCTTTTACTTATTTATCTTGTTCAGTTTCTGCCTGCCTCACCTGCCTCACCA
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| − | CAGTGTACATTTCACGAGGGCAGGGATTTTGTCTGTTTTGTTGATTGCTGTAGCCCTGGCATCTTGGGCA
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| − | GTGCCAGCACCTGTTAGCATTAAATACATATTTACTGAATGAATGAGTGGCTTCATGCTTAAAAGCAAGA
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| − | AGAGAGACCTTCATAGAGAAGTAGGAAAATGAATTGTTGCACAAATAAAGGGCCATGTAATGAGAGAGAG
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| − | AGAGCAAGAGAAAGAGCAAACAATTAAGGAGCAGCAGTGGCTTCCTGCAGGGGAGACCAATGCCTTCTGT
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| − | CCTCAGCGGAAACAGCTTGGCATTCTTCCAATTGATGAATGGCATGGACCAGGAGCACTAGTTATCTTCA
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| − | GCTTCTGAGCATCCGAATAAATAAACATACAAATGCATAACATAAAATAAATTCAAATTACAAATGAGAA
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| − | AACCAATCTAGGTTACCGGCAAGTCCTATGACTTGATGTGGAGGCCTGGAAGTGCATCCTTGAATCTTGC
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| − | AATGCAGTGAGCCAAGGAGCAATGAGAGGCCACATGAATAACCGGGCCAGTGACAAGACACATGTCCTCC
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| − | TCCTTGCTGGTATACACCAGAATGCCCCGCATGCAGCGAATGCTCAAAAAGTGTTGGTGGAAGTGACAGA
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| − | ATTCCATCACCATTAAACACAACCAGTGTTGCGGTTGACAATGGCACTGAAGCAGGTGAAAAATCCGTAC
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| − | AGCAGCTTCTGTCTTTGAAGAGTTTGCAGCCTCTTGAAGAGGGTTTAGAGGTTAAAAAAAGGACCCAGGC
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| − | TGATAGGGCCGCTACCATTTGGAAAGTGACAAGAGGAAGAGAGAGAATGAAACAACAGTAAGCACTGGCT
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| − | CTCAGCTTCTACCTGAAGAGACAAACATTGCTTTCACTTACATTTCACTGGCCAAATCAAGCCCATGGCA
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| − | ACAATTGAGTTCAAAAGCATAAGGAGGTATCTGGAAGGAAGAGAATCAGAAAATTTCTGATGAGCCTCAC
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| − | TGACTACCTTCAGGGCAAAAAGGAAGAGAGGCACTCCAGATCAGAGATCTGCATTGGAAACTCCTTGAGA
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| − | ACCAGAAGGGTATGCTGTATTTTTGTAACTGTGAGGAGCCCAGAAAGACTGAAGCCCAAATAAAATTCCT
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| − | TTATTCAATATT
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| − | </dnaseq>
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| − |
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| − | ==Labs working on this lncRNA==
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| − | * Department of Molecular Virology, Immunology and Medical Genetics andDepartment of Anesthesiology, Wexner Medical Center, and Department of Neurological Surgery, The Ohio State University, Columbus, OH, 43210; <ref name="ref1" />.
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| − | ==References==
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| − | <references>
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| − | <ref name="ref1"> Kim T, Cui R, Jeon YJ, Lee JH, Lee JH, Sim H, et al. Long-range interaction and correlation between MYC enhancer and oncogenic long noncoding RNA CARLo-5[J]. Proceedings of the National Academy of Sciences of the United States of America. 2014,111(11):4173-8</ref>(1)
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| − | <ref name="ref2"> Ma G, Gu D, Lv C, Chu H, Xu Z, Tong N, et al. Genetic variant in 8q24 is associated with prognosis for gastric cancer in a Chinese population[J]. Journal of gastroenterology and hepatology.</ref>(2)
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| − | </references>
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| − | [[Category: Antisense]]
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