NONHSAT129019

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PRNCR1(PCNCR1), is pervasively transcribed from the 8q24 “gene desert” region in strong association with susceptibility of prostate cancer.

Contents

Annotated Information

Name

PRNCR1: prostate cancer associated non-coding RNA 1(HGNC nomenclature)

pubmedID

20874843 23945587 24330491

Disease

Prostate Cancer

Characteristics

PRNCR1(prostate cancer non-coding RNA 1) , ∼13 kb intron-less long non-coding RNA (ncRNA) ,was transcribed in centromeric region (so-called Region 2: Chr8: 128.14-128.28 Mb) on 8q24 hat ,a large “gene-desert” ,whose region is susceptibility to prostate cancer[1].

PRNCR1 (also known as PCAT8) and PCGEM1, bind successively to the androgen receptor and strongly enhance both ligand-dependent and ligand-independent androgen-receptor-mediated gene activation programs and proliferation in prostate cancer cells[2].

Function

PRNCR1 overexpression can potentially serve as a required component of castration-resistance in prostatic tumors[2].

the polymorphisms in the lncRNA PRNCR1 may be related to the development of CRC, offering a novel and potential strategy for functional analysis of susceptibility loci to human diseases[3].

Diseases

Expression

PRNCR1 expression was upregulated in some of the PC cells as well as precursor lesion prostatic intraepithelial neoplasia[1].

The primers for real-time quantitative PCR were as follows,

Primer Forward Reverse
PRNCR1 5'-CCAGGG-GGAAACACACAG-3' 5'-AAATGGCAGTTTCCTTCAATG-3'[1]
β-actin (ACTB) 5'-TTGGCTTGACTCAGGATTTA-3' 5'-ATGCTATC-ACCTCCCCTGTG-3'[1]

siRNA target sequences of were as follows,:

Primer Sequences
si1 5'-GCUCUUAAGGAAAUAACUU-3'[1]
si2 5'-GACGGUUCUGUUUAAGUAA-3'[1]
siEGFP(as a negative control) 5'-GAAGCAGCACGACUUCUUC-3'[1]

The knockdown effect was measured by RT-PCR using the primer set: 5′-ATTGTTCCCAGTTCTCTGGAC-3′ and 5′-GGAGGAAGTGGAGTAGGTATAAGAG-3′.

Allelic Information and Variation

The transcript is an approximately 13-kb intron-lessnon-coding RNA spanning a region between rs1016342 andrs7463708, and named this novel ncRNA as PRNCR1(prostate cancer non-coding RNA 1, GenBank Accession no.AB458446).[1]

Labs working on this lncRNA

  • Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan[1].
  • Howard Hughes Medical Institute, Department of Medicine, University of California San Diego, La Jolla, California 92093, USA[2].
  • Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children's Health; Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P,R, China[3].

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Chung S, Nakagawa H, Uemura M, Piao L, Ashikawa K, Hosono N, et al. Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility[J]. Cancer science. 2011,102(1):245-52.
  2. 2.0 2.1 2.2 2.3 Yang L, Lin C, Jin C, Yang JC, Tanasa B, Li W, et al. lncRNA-dependent mechanisms of androgen-receptor-regulated gene activation programs[J]. Nature. 2013,500(7464):598-602.
  3. 3.0 3.1 3.2 Li L, Sun R, Liang Y, Pan X, Li Z, Bai P, et al. Association between polymorphisms in long non-coding RNA PRNCR1 in 8q24 and risk of colorectal cancer[J]. Journal of experimental & clinical cancer research : CR. 2013,32:104.

Basic Information

Transcript ID

NONHSAT129019

Source

NONCODE4.0

Same with

PRNCR1,

Classification

intergenic

Length

1248 nt

Genomic location

chr8-:128098508..128099755

Exon number

1

Exons

128098508..128099755

Genome context

Sequence
000001 CAGCAGAGAC GCCAATACAG ACGACAAAAC CAGCAGTCTT CATCTGACTC TGGTTCCTCC TTCTCCTCAG AAGATGAACG 000080
000081 ACCCAAGAGA TCCCATGTGA AGAATGGTGA GGTTGGCAAG CGGCGGAGAC ATTCCCCTTC CCGGAGTGCT TCTCCATCAC 000160
000161 CACGAAAGCA CCAAAAAGAG ACTTCCCCTC ATGGTAGACG GAGGAGAAGT CCATCCCCAC CACCCACCAG AAGGCGACGG 000240
000241 TCTCCTTCTC CCGCCCCTCA TCCTCGACGG CTCAGGACTC CCACACCACC ACCACCACGA CGAAGGACTC CTTCTCCTCC 000320
000321 CCTACGTCGG CGCTCACCTT CTCCTAGAAG ATACTCTCCT CCAATACAGA GGAGATACTC TCCTTCTCCA CCTCCAAAGA 000400
000401 GAAGAACGGC TTCACCTCCT CCCCCTCCTA AACGAAGAGC ATCACCATTT CCACCACCAA AGCGGCGGGT CTCCCATTCT 000480
000481 CCACCTTCCA AAGAAGCTCC CCAGTCACCA AGAGACGTTC ACCTTCACTA TCATCCAAGC ATAGGAAAGG GTCTTCCCCA 000560
000561 AGCCGCTCTA CCCGGGAGGC CCGATCACCG CATCCAAACA AATGGCATTC GCCCTCATCA CGGCCTCGAG CTCCTCAGAC 000640
000641 CTCAAGTCCT CCACCCGTTC GAAGAGGAGC GTCGTCATCA CCCCAAAGAA GGCAGTCCCC GTCTCCAAGT ACTAGGCCCA 000720
000721 TTAGGAGAGT CTCCAGGACT CCGGAACCTA AAAAGATAAA AAAAGGCTGC TTCCCCAAGC CCACAGTCTG TAAGGAGGGT 000800
000801 CTCATCCTCC CGATCTGTCT CTGGGTCTCC TGAGCCAGCA GCTAAAAAGC CCCCAGCATC TCCATCCCCC ATCCAGTCTC 000880
000881 AGTCACCGTC TACAAACTGG TCACCAGCTG TACCGGTCAA AAAGGCCAAA AGCCCAACAC CGAGCCCATC ACTGCCAAGA 000960
000961 AATTCAGATC aagaaggaga tggaaagaaa aagaagaaaa agaaggacag gaaacacaaa aaggataaga agcacaagaa 001040
001041 gcacaaaaaa cacaagaagg aaaaggctgt ggctgcagct gctgcagctg ctgtgacccc tgcagccatt gcagctgcCA 001120
001121 CAACCACATT AGCACAGGAA GAGCCAGTGG CAGCGCCAGA GCCGAAGAAG GAGACTGAAA GTGAAGCTGA AGATAACCTT 001200
001201 GATGATTTAG AAAAGCACCT GCGTGAAACG GCCCCGAGAT CAATGAGG
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