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Zfas1: Zinc finger antisense 1
Expressed from a bi-directional promoter with the protein-coding gene Znfx1. Zfas1 5°Ø is antisense to 5°Ø of Znfx1. Spliced, polyadenylated. SnoRNA host gene, contains 3 intronic C/D box snoRNAs. Human orthologue is alternatively spliced.
Zfas1 appears to have a role in mammary gland proliferation and differentiation. - Zfas1 knockdown in a HC11 cell culture model of mammary differentiation showed increased differentiation and a probable increase in proliferation. Knockdown had little effect on intronic snoRNA expression suggesting the Zfas1 host transcript is functional. - Zfas1 knockdown appears to affect Znfx1 expression.
Highly expressed and differentially expressed during mammary development. Strongly down-regulated during lactation compared to pregnancy and then up-regulated again during involution. Znfx1 coding gene,in contrast, was more lowly expressed and was not differentially expressed during mammary gland development. Expressed in the epithelial cells of the mammary gland ducts and alveoli. Zfas1 is widely expressed in mouse tissues including brain, liver, lung, kidney, spleen, heart and embryo, showing higher expression than Znfx1. Zfas1 extremely stable with a half life of >32 hrs in N2A (neuroblastoma) cells. Compared to a half life of 50 minutes for Znfx1. The increased stability of Zfas1 may explain why its expression level is much higher than Znfx1. However, in mouse 3T3 and embryonic stem cells Zfas1 stability was much lower with a half-life of 1.7 hr and 63 min respectively, while the human ZFAS1 had a half-life of 3 hr in B-cells (Friedel (2009), Sharova (2009), Clark (2012)). These results suggest there is significant regulation of Zfas1 stability both within and between species (Clark (2012)). Localised to both the nucleus and the cytoplasm. Human ZFAS1 expression is reduced in ductal carinoma compared to normal tissue.
Syntenic transcription observed in multiple mammals. Low conservation of Zfas1 sequence between human and mouse compared to snoRNAs, but Zfas1 has potential secondary structural similarities.
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Allelic Information and Variation
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Labs working on this lncRNA
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