Approved name: SPRY4 intronic transcript 1 (non-protein coding)
LncBook ID: HSALNT0289259
Single exon ~700 nt transcript expressed in sense direction from the 2nd intron in SPRY4. Contains a SINE at 5' end. Secondary structure predicted to contain several long hairpins and multiple pseudoknots.
Knockdown of SPRY4-IT1 in melanoma cells lines decreased cell viability, increased cell apoptosis. SPRY4-IT1 impacts cell invasion and cell mobility with decreased invasion and motility in SPRY4-IT1 knockdown cells and increased mobility (faster speed of migration) in SPRY4-IT1 over-expressing cell lines. Conservation Not well conserved outside of primate, with 5' SINE element and predicted secondary structural elements only present in primates.
Expression significantly up-regulated in melanoma cell lines and patient tumour samples (both primary and metastatic) compared to melanocytes and keratinocytes. SPRY4-IT1 showed much greater expression variation across 20 human tissue than SPRY4. Both showed positively correlated expression and depending on the tissue either could be more highly expressed than the other. Knockdown of SPRY4-IT1 did not affect expression levels of SPRY4, validating that the two transcripts are independent. Localized predominately in the cytoplasm. Larger punctate nuclear signal could be transcription sites.
Not well conserved outside of primate, with 5' SINE element and predicted secondary structural elements only present in primates.
SPRY4 protein coding gene is also more highly expressed in melanoma cells and patient samples compared to melanocytes. It is unclear if SPRY4-IT1 has a separate promoter or is transcribed as part of the SPRY4 transcript.
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Allelic Information and Variation
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Labs working on this lncRNA
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