Lnc-HEATR6-1:4

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WFDC21P, locus in 17q23.1 and spanning 621 bp in length, bounds directly to STAT3 in the cytoplasm and regulates DC differentiation.

Contents

Annotated Information

Name

WFDC21P: WAP four-disulfide core domain 21, pseudogene(HGNC nomenclature)

lnc-DC[1]

Characteristics

lnc-DC has two transcript variants(417 and 397 nucleotides), of which the 417-nt variant was expressed more highly[1].

lnc-DC has poly A tail and 5’cap structure but is without coding capacity[1].

A canonical binding motif(+44 to 50 nt relative to TSS) for the transcription factor PU.1-a key regulator of DC differentiation was found in lnc-DC promoter region[1].

Cellular Localization

Function

Proposed model for lnc-DC in human DC differentiation.[1]

lnc-DC bounds directly to STAT3 in the cytoplasm which promotes STAT3 phosphorylation on tyrosine-705 by preventing STAT3 bing to and dephosphorylation by SHP1[1].

lnc-DC is vital for DC differentiation in both human and mice and function by directly interacting with signaling molecules in the cytoplasm and regulating their posttranslational modification[1]. .

Regulation

PU.1 directs lnc-DC expression in human cDCs[1].

Diseases

  • DC dysfunction[1]

Expression

lnc-DC is exclusively expressed in cDCs of the hematopoietic system[1].

lnc-DC is more exclusively expressed in human cDC than other DC markers.[1]

Knockdown and Overexpression:

Primer Sequences
RNAi-1 5'- GAG TTA TCT TAA GGA TCA T -3'[1]
RNAi-2 5'- GGA GTT CCT TGA CTA GG -3'[1]
scrambled control 5'- TTC TCC GAA CGT GTC ACG T-3'[1]


Labs working on this lncRNA

  • National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, China[1].

References

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Wang P, Xue Y, Han Y, Lin L, Wu C, Xu S, et al. The STAT3-binding long noncoding RNA lnc-DC controls human dendritic cell differentiation[J]. Science. 2014,344(6181):310-3.</ref(1)>

Basic Information

Transcript ID

lnc-HEATR6-1:4

Source

LNCipedia2.1

Same with

NONHSAT055064, WFDC21P, lnc-DC

Classification

intergenic

Length

621 nt

Genomic location

chr17-:58160927..58165828

Exon number

3

Exons

58160927..58161127,58162416..58162564,58165558..58165828

Genome context

Sequence
000001 AGGACCCAGG GTACACAGGG TGGGTGGCTA TTCTCCAGAA ATGTCAGTTT CTGGGCAGGG CTTAGGTGTC TGCAGTCCCT 000080
000081 AGTCCCACCC CTGGCCTTGC ATTCCAGCTC AGCGGGTGGA AGGTATAAAT TTCAGCTGCT CTCAGCCCTG CTGTGTTTTT 000160
000161 CCAAAGCCTT CCAACAGCAA CATGAAGTTG GCAGCCTTCC TCCTCCTGTG ATCCTCATCA TCTTCAGCCT AGAGGTACAA 000240
000241 GAGCTTCAGG CTGCAGGAGA CCGGCTTTTG GGTACCTGCG TCGAGCTCTG CACAGGTGAC TGGGACTGCA ACCCCGGAGA 000320
000321 CCACTGTGTC AGCAATGGGT GTGGCCATGA GTGTGTTGCA GGGTAAGGAC AGGTAAAAAC ACCAGGCCCT CCCTGCTTTC 000400
000401 TGAAACGTTG TTCAGTCTAG ATGAAGAGTT ATCTTAAGGA TCATCTTTCC CTAAGATCGT CATCCCTTCC TGGAGTTCCT 000480
000481 ATCTTCCAAG ATGTGACTGT CTGGAGTTCC TTGACTAGGA AGATGGATGA AAACAGCAAG CCTGTGGATG GAGACTACAG 000560
000561 GGGATATGGG AGGCAGGGAA GAGGGGTTGT TTCTTTTAAT AAATCATCAT TGTTAAAAGC A
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