WFDC21P, locus in 17q23.1 and spanning 621 bp in length, bounds directly to STAT3 in the cytoplasm and regulates DC differentiation.
WFDC21P: WAP four-disulfide core domain 21, pseudogene(HGNC nomenclature)
lnc-DC has two transcript variants(417 and 397 nucleotides), of which the 417-nt variant was expressed more highly.
lnc-DC has poly A tail and 5’cap structure but is without coding capacity.
A canonical binding motif(+44 to 50 nt relative to TSS) for the transcription factor PU.1-a key regulator of DC differentiation was found in lnc-DC promoter region.
lnc-DC bounds directly to STAT3 in the cytoplasm which promotes STAT3 phosphorylation on tyrosine-705 by preventing STAT3 bing to and dephosphorylation by SHP1.
lnc-DC is vital for DC differentiation in both human and mice and function by directly interacting with signaling molecules in the cytoplasm and regulating their posttranslational modification. .
PU.1 directs lnc-DC expression in human cDCs.
- DC dysfunction
lnc-DC is exclusively expressed in cDCs of the hematopoietic system.
Knockdown and Overexpression:
|RNAi-1||5'- GAG TTA TCT TAA GGA TCA T -3'|
|RNAi-2||5'- GGA GTT CCT TGA CTA GG -3'|
|scrambled control||5'- TTC TCC GAA CGT GTC ACG T-3'|
Labs working on this lncRNA
- National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Wang P, Xue Y, Han Y, Lin L, Wu C, Xu S, et al. The STAT3-binding long noncoding RNA lnc-DC controls human dendritic cell differentiation[J]. Science. 2014,344(6181):310-3.</ref(1)>
NONHSAT055064, WFDC21P, lnc-DC