File:ANCR regulates a global gene expression program associated with epidermal differentiation..jpg

(A) Increased mRNA expression of early and late differentiation genes in ANCR-depleted progenitor keratinocytes in cultured keratinocytes versus control siRNAs, as shown by qPCR analysis. (B) Heat map depicting transcript profiling of independent biologic replicate progenitor keratinocytes with siRNA-mediated ANCR depletion (ANCRi) versus control siRNA (CONTROLi). Expression changes for each probe are normalized to a mean-centered signal across all samples. (C) Gene ontology terms significantly enriched in the ANCR-depleted gene set. (D) Gene set enrichment analysis (GSEA) shows highly statistically significant overlap of the ANCRi gene set with a calcium-induced keratinocyte differentiation gene signature published previously (Sen et al. 2010). (E) Gene set overlap of ANCRi gene set with published gene signatures from loss of key transcription factors that are dysregulated by ANCR depletion. Black bars indicate significantly differentially expressed genes in the gene set. The 1000 genes total between these four gene sets are compared with each other as well as with the keratinocyte differentiation signature. The total number of genes in each gene signature falling within the 1000-gene set is listed to the right of each row.