ENST00000433810.1

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lncUSMycN,a 1040 bp long non-coding RNA,locating in proximity (14kb upstream) to the MYCN transcription start site,contributes to tumorigenesis.

Contents

Annotated Information

Name

MYCNUT: MYCN upstream transcript (non-protein coding)(HGNC nomenclature)

lncUSMycN[1]

Charicteristics

The lncUSMycN gene, which is located in proximity (14kb upstream) to the MYCN transcription start site.[1].

Function

Binding of lncUSMycN to the RNA-binding protein NonO[1]

LncUSMycN RNA bounding to the RNA-binding protein NonO, leading to N-Myc RNA upregulation, which in turns leads to post-transcriptional upregulation of N-Myc proteian expression and induces the proliferation of neuroblastoma cell, may contributes to tumorigenesis[1].

Diseases

  • Neuroblastoma, tumorigenesis[1]

Expression

Long noncoding RNA USMycN (lncUSMycN) is frequently coamplified with the 130-kb amplicon containing MYCN and consecutively overexpressed in MYCN oncogene–amplified neuroblastoma[1].

LNA-ASO targeting lncUSMycN (5’-TGAGGGTTGAGTTGGT-3’) and negative control LNA-ASO (5’-GACGGTAACTAGGCGA-3’) were designed and synthesized by Exiqon (Exiqon, Vedbaek, Denmark).

Specific primers in RT-PCR used as follows,

Primer Forward Reverse
lncUSMycN 5'-ACAAATGAGGCAACAAGCAACTT-3' 5'-CTTTGGTGCTCAAGCCTTCAG-3'[1]
β-actin 5'-CACCATGTACCCTGGCATT-3' 5'-ACGGAGTACTTGCGCTCAG-3'[1]

Labs working on this lncRNA

  • Children's Cancer Institute Australia for Medical Research, Randwick NSW, Australia[1].

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Liu PY, Erriquez D, Marshall GM, Tee AE, Polly P, Wong M, et al. Effects of a novel long noncoding RNA, lncUSMycN, on N-Myc expression and neuroblastoma progression[J]. Journal of the National Cancer Institute. 2014,106(7).

Basic Information

Transcript ID

ENST00000433810.1

Source

Gencode19

Same with

lnc-MYCN-1:1,NONHSAT069300,MYCNUT, lncUSMycN

Classification

intergenic

Length

1039 nt

Genomic location

chr2+:16060521..16076139

Exon number

3

Exons

16060521..16061185,16061690..16061819,16075896..16076139

Genome context

Sequence
000001 ACTAAACCAG GACACTTTTG AGAATGAACT TGGGTGCTAT TGGTAATCAC ACCGAGACAC AGCAACGTCC GGGACCAACT 000080
000081 CAACCCTCAG TCCGCTCATG GGGCACAGGT TTCCTGGTCC TTCCCTACCC TGACTTCTCT TCTTCTGATG TTTGGTGGTC 000160
000161 TGCATCCTTT TAAACCACTG TGCTCCCAGA ACTGATTCCC ATATGTGTCT GCCATACTGG AAAATACCAA ATAGGCAAGG 000240
000241 AGATCGTTTC AATACTGTCT GCTGAACACC TTAATAGAGG CAAGAGAGAA CGCAGAGGAA GAAACAATGG ACTCTTTCTG 000320
000321 AGAGAGTGGA AGATGGCTTC GCAGAGGAGC TGCTGTCTGA GACGGATAGC ATGAGTAGGA GTTTCCCCAG GCAGGGAAGG 000400
000401 TGTTGTTGTT TCTCCTCAGG GTAGAGGGGA GAGCAGGGTT CCCCGTGTGT CTGTTGCTGA ATGTGACTCA TGCTGTTTTC 000480
000481 CTGCTTTGAG CCTGCTCTAG GGTGTTATGG GCAGGATGGA CTTGTCCTGC GTGCTTGTTG TTCCGCCGTG TTTCTGATCT 000560
000561 TCAGATTTAT CGTAAGGAAG AACAGCCGAT GTACAGGCAG AACTTGACAA ATGAGGCAAC AAGCAACTTG ATCTGGGATC 000640
000641 CATCGTCCTC TAGACCCCAG TAGGAGTCTA AGCACCAGCG TGGTGGCCAC CCCAGCAGCC TTGGACCCCT GAAGGCTTGA 000720
000721 GCACCAAAGC ACACACAGCT GCCTCTGGCT GAGTTCTGTG GTCGCCTGCA CCCCCTCCAC CTGCTTCCCC CGGAGGATGG 000800
000801 ATCATGAAGA GTGGAATTGC TTTTCCTCTT GGGGCAGGCA CACCAACAGT GTATGTGAGG CCCCTGCTCC CACTCCCCAG 000880
000881 GCTGGCCAGT ACTTTGGGCC AATCTGCTAG ACCCAAAAAT GTTAATCTCA CTGAGATTTA ATTTGCATTC CCCTGACTAC 000960
000961 CGGTGAAGTT GAGCATCTTT TCATATGTTT ATTGACAATT TGCATTTCTT ATTCAGTAAA TTACCTGCTC GTATCCTAG
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