lncUSMycN,a 1040 bp long non-coding RNA,locating in proximity (14kb upstream) to the MYCN transcription start site,contributes to tumorigenesis.
MYCNUT: MYCN upstream transcript (non-protein coding)(HGNC nomenclature)
The lncUSMycN gene, which is located in proximity (14kb upstream) to the MYCN transcription start site..
LncUSMycN RNA bounding to the RNA-binding protein NonO, leading to N-Myc RNA upregulation, which in turns leads to post-transcriptional upregulation of N-Myc proteian expression and induces the proliferation of neuroblastoma cell, may contributes to tumorigenesis.
- Neuroblastoma, tumorigenesis
Long noncoding RNA USMycN (lncUSMycN) is frequently coamplified with the 130-kb amplicon containing MYCN and consecutively overexpressed in MYCN oncogene–amplified neuroblastoma.
LNA-ASO targeting lncUSMycN (5’-TGAGGGTTGAGTTGGT-3’) and negative control LNA-ASO (5’-GACGGTAACTAGGCGA-3’) were designed and synthesized by Exiqon (Exiqon, Vedbaek, Denmark).
Specific primers in RT-PCR used as follows,
Labs working on this lncRNA
- Children's Cancer Institute Australia for Medical Research, Randwick NSW, Australia.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Liu PY, Erriquez D, Marshall GM, Tee AE, Polly P, Wong M, et al. Effects of a novel long noncoding RNA, lncUSMycN, on N-Myc expression and neuroblastoma progression[J]. Journal of the National Cancer Institute. 2014,106(7).